FastQCFastQC Report
Thu 26 May 2016
SRR1512827_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512827_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039774
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51030.2501747742642077No Hit
GTCCTACAGTGGACATTTCTAAATT41260.20227731111387828No Hit
GTCCTAAAGTGTGTATTTCTCATTT36920.18100044416685376No Hit
CTGTAGGACGTGGAATATGGCAAGA36500.17894139252681915No Hit
CTTTAGGACGTGAAATATGGCGAGG32690.16026285264936213No Hit
GGTATCAACGCAGAGTACTTTTTTT31220.15305617190924092No Hit
TATCAACGCAGAGTACTTTTTTTTT30340.1487419684729779No Hit
GTCCTACAGTGTGCATTTCTCATTT23950.11741496852102243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7450.013.0012574
TAGTACC853.963529E-612.2889614
CCAACGA1052.743982E-711.75436219
GGCGAGG10550.011.69865419
TATGTCG907.44514E-611.61369116
GCCGGTT1001.9487616E-611.39100311
GGTATCA12350.011.3668431
GTAGGAC23600.011.26757053
AGGACGT23200.011.25577455
TGTAGGA25100.011.237432
GGACGTG23150.011.1980496
TAGGACA6400.011.1281434
TCCAACG1803.6379788E-1211.07567918
TTAGGAC13900.011.0683913
ATGTCGA951.3599043E-511.00054317
TGTCGAG951.3711078E-510.99240218
TAGGACG24850.010.8908014
GACGTGA12400.010.7207787
CTAGTAC803.7829374E-410.6840723
GTCCTAC21950.010.5569021
Produced by FastQC (version 0.11.5)