##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512827_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2039774 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.06568031556437 31.0 25.0 34.0 10.0 34.0 2 28.591964599999805 31.0 28.0 34.0 16.0 34.0 3 29.754828721221077 31.0 30.0 34.0 25.0 34.0 4 33.58762441329284 37.0 35.0 37.0 28.0 37.0 5 33.95213097137232 37.0 35.0 37.0 30.0 37.0 6 34.22752275497187 37.0 35.0 37.0 31.0 37.0 7 34.20471287505381 37.0 35.0 37.0 31.0 37.0 8 34.24721758390881 37.0 35.0 37.0 31.0 37.0 9 35.85633555482127 39.0 37.0 39.0 31.0 39.0 10 35.78034968579853 39.0 37.0 39.0 31.0 39.0 11 35.83187058958492 39.0 37.0 39.0 31.0 39.0 12 35.74226752571609 39.0 37.0 39.0 30.0 39.0 13 35.737136565129276 39.0 37.0 39.0 30.0 39.0 14 36.95865277231693 40.0 38.0 41.0 31.0 41.0 15 36.88465143687487 40.0 38.0 41.0 31.0 41.0 16 36.79360507585644 40.0 38.0 41.0 30.0 41.0 17 36.75565626388021 40.0 38.0 41.0 30.0 41.0 18 36.6836463255243 40.0 38.0 41.0 30.0 41.0 19 36.710610097000945 40.0 38.0 41.0 30.0 41.0 20 36.634587949449305 40.0 38.0 41.0 30.0 41.0 21 36.55080856996903 40.0 38.0 41.0 30.0 41.0 22 36.43230573583152 40.0 38.0 41.0 30.0 41.0 23 36.302238385232876 40.0 37.0 41.0 29.0 41.0 24 36.19864651672195 40.0 37.0 41.0 29.0 41.0 25 36.089756021990674 40.0 37.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.534791172358741 1101 2 2.107496774243554 1101 3 1.0518953547917356 1101 4 0.7085451840771455 1101 5 0.3248131166178183 1101 6 0.20456251694134409 1101 7 0.056538250602699236 1101 8 -0.05431123428297724 1101 9 0.045004014653343916 1101 10 0.010060685662672597 1101 11 -0.024477908398495174 1101 12 0.06813141172613513 1101 13 0.00840654432047927 1101 14 -0.3587444306707184 1101 15 -0.43244814555585265 1101 16 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1.093158139310205 1103 3 0.0987158250703537 1103 4 -0.3909766230437839 1103 5 -0.5577364190154199 1103 6 -0.8720627667323981 1103 7 -0.9270086723715423 1103 8 -0.9828898142932729 1103 9 -1.4065664413224042 1103 10 -1.4284917677576203 1103 11 -1.417740299458245 1103 12 -1.4146336205983658 1103 13 -1.297172054760324 1103 14 -1.776941505255465 1103 15 -1.819536461058263 1103 16 -1.7019553522815585 1103 17 -1.9270640976542168 1103 18 -1.726085320096118 1103 19 -1.6839814972919598 1103 20 -1.7704885070336545 1103 21 -2.0029133862825503 1103 22 -1.8869358863245367 1103 23 -2.0497018068785096 1103 24 -2.0175946738689063 1103 25 -1.8184856676994912 1104 1 -6.8270900946855235 1104 2 -8.332271297919235 1104 3 -7.951039153284626 1104 4 -6.213765367632529 1104 5 -4.123417804296803 1104 6 -2.961309519979153 1104 7 -2.366082265445133 1104 8 -1.9513218489253106 1104 9 -2.2583136274695903 1104 10 -2.00105540412126 1104 11 -1.8475619444798923 1104 12 -2.0071444431091905 1104 13 -2.178198028786298 1104 14 -2.220806440320395 1104 15 -2.2670983658201678 1104 16 -2.4783700708962755 1104 17 -2.659738556528673 1104 18 -2.4328186534294503 1104 19 -2.4477745708850307 1104 20 -2.479050411795555 1104 21 -2.4439679317370917 1104 22 -2.5566735486622036 1104 23 -2.757711330688032 1104 24 -2.858644890319127 1104 25 -2.7894640226778478 1105 1 3.119151610660875 1105 2 1.8340918445940915 1105 3 0.5957562395295426 1105 4 0.41920943037331426 1105 5 -0.1047420607863998 1105 6 -0.16510666627449666 1105 7 -0.45574497818365955 1105 8 -0.3081570560767659 1105 9 -0.4670474765326773 1105 10 -0.591952705135526 1105 11 -0.5051721215471048 1105 12 -0.5672544654574807 1105 13 -0.74685128647927 1105 14 -0.9978926359538818 1105 15 -1.071771290007895 1105 16 -1.0104144924194003 1105 17 -0.8788257836216786 1105 18 -0.9714652410175546 1105 19 -0.8358580503417983 1105 20 -1.1877783471574261 1105 21 -1.201582647226175 1105 22 -1.3152396824081194 1105 23 -1.3775555115372669 1105 24 -1.343787884989915 1105 25 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2314 14 -0.29353921963711116 2314 15 -0.22138977856255337 2314 16 0.018049504357648516 2314 17 -0.006044743047603163 2314 18 0.08709565866658409 2314 19 -9.032874131946755E-4 2314 20 0.29693333705024116 2314 21 0.13062560473566975 2314 22 -0.32311934736949866 2314 23 -0.08983173696688596 2314 24 0.013485118914488226 2314 25 0.06420910751605646 2315 1 0.17770784611241908 2315 2 1.1922718634239295 2315 3 0.9770503284048573 2315 4 0.671662314595153 2315 5 0.5850914738124757 2315 6 0.31069141602178263 2315 7 0.4287594333965643 2315 8 0.2857570793536155 2315 9 -0.5473175820735392 2315 10 -0.2984315126973698 2315 11 -0.107748489466438 2315 12 -0.03739599336073951 2315 13 -0.053650936239208136 2315 14 0.02626429795034113 2315 15 0.20225481073300955 2315 16 0.07300954768334122 2315 17 0.12694608135596752 2315 18 0.0785098594990643 2315 19 0.04526560695514803 2315 20 0.10968874814360419 2315 21 0.09416746079830318 2315 22 -0.6774910518797057 2315 23 -0.2690039011806036 2315 24 0.02579774452350847 2315 25 0.20000035078652445 2316 1 1.3131660423666034 2316 2 1.0392855390179854 2316 3 0.43184888599250826 2316 4 0.28860939471992 2316 5 0.020029307371430605 2316 6 -0.19858853387239606 2316 7 -0.21743419274065445 2316 8 -0.20545927710874423 2316 9 -0.15544213472959 2316 10 -0.17272388839995756 2316 11 -0.2796740932315913 2316 12 -0.2864758961542151 2316 13 -0.28481706148242125 2316 14 -0.35464720079255585 2316 15 -0.37824697127300766 2316 16 -0.1907178824496043 2316 17 -0.039508492807790674 2316 18 -0.06327063295519508 2316 19 -0.11450291100829446 2316 20 -0.09924236834000766 2316 21 -0.1196360817423141 2316 22 -0.029073506878020794 2316 23 -0.14518426498240444 2316 24 -0.15255267262352135 2316 25 -0.2361402997069959 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 66610.0 3 5.0 4 613.0 5 2493.0 6 3060.0 7 2818.0 8 2417.0 9 2344.0 10 2360.0 11 2503.0 12 2513.0 13 2595.0 14 2504.0 15 2501.0 16 2554.0 17 2630.0 18 3136.0 19 3844.0 20 4841.0 21 5485.0 22 6794.0 23 7910.0 24 9001.0 25 10458.0 26 11910.0 27 13615.0 28 16257.0 29 19376.0 30 24500.0 31 32126.0 32 43122.0 33 59694.0 34 87211.0 35 132600.0 36 241727.0 37 341590.0 38 864057.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.40958279724325 21.050487218615043 11.922593578887525 23.617336405254182 2 14.229623772036462 20.214237098760332 42.35367710411085 23.202462025092355 3 18.476989932942008 24.961327824986405 29.98423611022332 26.57744613184827 4 11.884108283746963 16.91175060494469 39.26886392346399 31.935277187844363 5 12.689722049974256 38.238622086216 36.218477188113305 12.85317867569645 6 31.270912047796156 37.76707233787482 18.348114046508883 12.613901567820134 7 27.618710214974023 31.74603018180331 22.64734040535087 17.987919197871793 8 24.59361731094659 37.819184831197525 20.716338419844323 16.870859438011557 9 26.452314780236414 15.099926489954282 20.539276771218656 37.90848195859065 10 14.664917065051478 29.22651854988747 35.21312338768987 20.895440997371175 11 33.95804674218666 22.370057792502358 25.319544520967046 18.352350944343936 12 23.198328593881932 24.734186074171348 32.44020968263533 19.627275649311397 13 28.980182100332634 22.240513416172945 25.98255088914373 22.79675359435069 14 20.459858726632053 22.588054941937504 28.078905409185868 28.873180922244586 15 22.553769413072146 31.724261592671173 23.547064523295685 22.174904470960996 16 21.51950795645304 28.389358256385776 28.700189566167666 21.390944220993514 17 21.316179425666263 29.016502170948982 28.392906014305336 21.274412389079426 18 21.178329937298297 27.57174072808697 30.655995988664042 20.59393334595069 19 23.193964180750264 27.15037608394089 28.019740045826865 21.635919689481984 20 23.354588853577667 27.539852872099353 28.682570473399643 20.422987800923334 21 22.572812907351388 26.89982537691532 27.669544258343787 22.85781745738951 22 22.82369473347041 28.531574231659988 27.83249959038595 20.812231444483643 23 21.8041787057223 28.17759647088609 28.426094400644665 21.592130422746944 24 22.863273199236612 27.03436583185446 29.065595152404928 21.036765816504 25 22.36522047226658 27.647057376181962 28.539871021079023 21.44785113047244 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1893.0 1 1893.0 2 1686.0 3 1479.0 4 1479.0 5 1479.0 6 2443.0 7 3407.0 8 3407.0 9 3407.0 10 4421.5 11 5436.0 12 5436.0 13 5436.0 14 8427.5 15 11419.0 16 11419.0 17 11419.0 18 19469.0 19 27519.0 20 27519.0 21 27519.0 22 42709.0 23 57899.0 24 57899.0 25 57899.0 26 82316.0 27 106733.0 28 106733.0 29 106733.0 30 136884.5 31 167036.0 32 167036.0 33 167036.0 34 186799.0 35 206562.0 36 206562.0 37 206562.0 38 220491.0 39 234420.0 40 234420.0 41 234420.0 42 244591.5 43 254763.0 44 254763.0 45 254763.0 46 259463.0 47 264163.0 48 264163.0 49 264163.0 50 251531.0 51 238899.0 52 238899.0 53 238899.0 54 209369.0 55 179839.0 56 179839.0 57 179839.0 58 152988.5 59 126138.0 60 126138.0 61 126138.0 62 102367.0 63 78596.0 64 78596.0 65 78596.0 66 60259.5 67 41923.0 68 41923.0 69 41923.0 70 30629.0 71 19335.0 72 19335.0 73 19335.0 74 13525.0 75 7715.0 76 7715.0 77 7715.0 78 5287.5 79 2860.0 80 2860.0 81 2860.0 82 1956.5 83 1053.0 84 1053.0 85 1053.0 86 726.0 87 399.0 88 399.0 89 399.0 90 275.0 91 151.0 92 151.0 93 151.0 94 114.0 95 77.0 96 77.0 97 77.0 98 68.5 99 60.0 100 60.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.6920501977179825 2 0.3101323970204542 3 0.29473853475924294 4 0.29988616385932954 5 0.30429841737368946 6 0.21884777431225225 7 0.5036342261446611 8 0.25380262715379254 9 0.22933913266861916 10 0.2089937414635151 11 0.24909622340514195 12 0.24904719836609351 13 0.26478423590064387 14 0.2865513532381529 15 0.4786314562299549 16 0.587614118034645 17 0.5813879380754927 18 0.27154969128932915 19 0.23154525942579912 20 0.22247562720183706 21 0.33454686646657916 22 0.36146161290417467 23 0.4694637739278959 24 0.35597080853074897 25 0.37548277407202957 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2039774.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.15968958082175 #Duplication Level Percentage of deduplicated Percentage of total 1 86.46133364419534 64.98406997441582 2 9.555402091788116 14.36362110077459 3 2.1344560594356494 4.8127516455326225 4 0.7377668514731794 2.2180131015897757 5 0.36677566852648974 1.3783372696124672 6 0.19065462541294967 0.8597725477911088 7 0.11509348583954478 0.6055273467932422 8 0.07512690878367503 0.45172121146781813 9 0.052971186001001945 0.3583168106906949 >10 0.2747021185820289 3.541197959411635 >50 0.018542971578691045 0.9553304174656231 >100 0.013688728236499243 2.098259360626432 >500 0.002038782350027753 1.066658661122831 >1k 0.00138111062421227 2.0541792474294276 >5k 6.576717258153668E-5 0.25224334527594305 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5103 0.2501747742642077 No Hit GTCCTACAGTGGACATTTCTAAATT 4126 0.20227731111387828 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3692 0.18100044416685376 No Hit CTGTAGGACGTGGAATATGGCAAGA 3650 0.17894139252681915 No Hit CTTTAGGACGTGAAATATGGCGAGG 3269 0.16026285264936213 No Hit GGTATCAACGCAGAGTACTTTTTTT 3122 0.15305617190924092 No Hit TATCAACGCAGAGTACTTTTTTTTT 3034 0.1487419684729779 No Hit GTCCTACAGTGTGCATTTCTCATTT 2395 0.11741496852102243 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.90250390484436E-5 0.0 4 0.0 0.0 0.0 9.80500780968872E-5 0.0 5 0.0 0.0 0.0 9.80500780968872E-5 0.0 6 0.0 0.0 0.0 9.80500780968872E-5 0.0 7 0.0 0.0 0.0 9.80500780968872E-5 0.0 8 0.0 0.0 0.0 9.80500780968872E-5 0.0 9 0.0 0.0 0.0 9.80500780968872E-5 0.0 10 0.0 0.0 0.0 1.470751171453308E-4 0.0 11 0.0 0.0 0.0 1.470751171453308E-4 0.0 12 0.0 0.0 0.0 1.470751171453308E-4 0.0 13 0.0 0.0 0.0 1.961001561937744E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 745 0.0 13.001257 4 TAGTACC 85 3.963529E-6 12.288961 4 CCAACGA 105 2.743982E-7 11.754362 19 GGCGAGG 1055 0.0 11.698654 19 TATGTCG 90 7.44514E-6 11.613691 16 GCCGGTT 100 1.9487616E-6 11.391003 11 GGTATCA 1235 0.0 11.366843 1 GTAGGAC 2360 0.0 11.2675705 3 AGGACGT 2320 0.0 11.2557745 5 TGTAGGA 2510 0.0 11.23743 2 GGACGTG 2315 0.0 11.198049 6 TAGGACA 640 0.0 11.128143 4 TCCAACG 180 3.6379788E-12 11.075679 18 TTAGGAC 1390 0.0 11.068391 3 ATGTCGA 95 1.3599043E-5 11.000543 17 TGTCGAG 95 1.3711078E-5 10.992402 18 TAGGACG 2485 0.0 10.890801 4 GACGTGA 1240 0.0 10.720778 7 CTAGTAC 80 3.7829374E-4 10.684072 3 GTCCTAC 2195 0.0 10.556902 1 >>END_MODULE