FastQCFastQC Report
Thu 26 May 2016
SRR1512827_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512827_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039774
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55010.26968673980548824No Hit
GTCCTACAGTGGACATTTCTAAATT45220.22169122657706197No Hit
CTGTAGGACGTGGAATATGGCAAGA42030.20605223912060844No Hit
GTCCTAAAGTGTGTATTTCTCATTT38860.19051130174225184No Hit
CTTTAGGACGTGAAATATGGCGAGG37940.18600099814979504No Hit
GGTATCAACGCAGAGTACTTTTTTT37500.1838438964316635No Hit
TATCAACGCAGAGTACTTTTTTTTT34470.1689893095999851No Hit
GTCCTACAGTGTGCATTTCTCATTT24070.11800326898960375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6750.013.3836584
TCGAACT655.4875032E-513.14529319
GGTATCA13150.012.6647921
TACGCTG550.003046259712.1029265
GGCGAGG9500.011.89226119
AGGGTCG600.00583342611.094355
TAGACAG3200.010.9952925
GAACAGT5050.010.720536
CCAACGA803.7945027E-410.6805519
GTCCTAC23400.010.6554361
AGGACCT14550.010.653195
TATAGGA3050.010.601472
TGGACGG909.422114E-510.5660475
AGGACGT22200.010.4946555
TCAGTAC2001.8189894E-1210.4603863
TGTAGGA22800.010.4277882
GGACGTG21500.010.4257266
GGACCTG14650.010.3732586
CTAGGAC2850.010.3436033
TAGGACT2950.010.3153274
Produced by FastQC (version 0.11.5)