FastQCFastQC Report
Thu 26 May 2016
SRR1512826_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512826_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891817
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47110.24901985762893555No Hit
GGTATCAACGCAGAGTACTTTTTTT28930.1529217678031226No Hit
TATCAACGCAGAGTACTTTTTTTTT24500.12950512655293825No Hit
GTCCTACAGTGGACATTTCTAAATT21670.1145459629551907No Hit
GTACATGGGAAGCAGTGGTATCAAC20680.10931289865774543No Hit
CTGTAGGACGTGGAATATGGCAAGA19470.10291693118309013No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT655.4839857E-513.1460786
TAGGACC5550.012.4881154
AATCCCG1251.4242687E-912.15281819
AAGCCGT550.003082464712.0837686
CAACCGT658.0646627E-411.6854036
GATATAC3000.011.5433681
ACTGCGT600.006045141711.0412628
TTAGGAC7000.010.9872423
GGACGTG11900.010.850736
TGTAGGA13400.010.8414742
GTACTAG803.3599162E-410.8219071
AGGACGT12100.010.7498465
GTAGGAC13000.010.6637793
GGAGCGT909.57376E-510.5493216
GTCTTAC1657.4760464E-1010.4939721
TAGGACG12500.010.405854
AGGACCT10600.010.3005165
GGTATCA15350.010.1521481
GGCGAGG6900.09.76958919
GACGTGG7300.09.7547687
Produced by FastQC (version 0.11.5)