FastQCFastQC Report
Thu 26 May 2016
SRR1512826_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512826_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891817
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56680.2996061458375731No Hit
GGTATCAACGCAGAGTACTTTTTTT37350.1974292439490712No Hit
TATCAACGCAGAGTACTTTTTTTTT34920.18458444976443283No Hit
GTCCTACAGTGGACATTTCTAAATT24750.1308266074361315No Hit
GTACATGGGAAGCAGTGGTATCAAC23250.12289772213697203No Hit
CTGTAGGACGTGGAATATGGCAAGA21860.11555028842641757No Hit
GTCCTAAAGTGTGTATTTCTCATTT20900.11047580183495549No Hit
CTTTAGGACGTGAAATATGGCGAGG19180.10138401335858596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5350.010.6619524
CTCGAAC909.571732E-510.54954918
GGTATCA17250.010.4810871
GTCCTAG2600.010.2477021
TTAGGAC8300.010.0796143
GTCCTAA7800.010.0037091
TCGAACT1054.1312436E-59.94671819
TGGCGAG13500.09.91657618
GGCGAGG6000.09.81108119
GACGTGA7550.09.6868137
GTCCTAC11300.09.5157241
GTATAAC1201.6674909E-59.5157231
TAGGTTG1401.0477106E-69.507165
AGGACCG1106.78067E-59.5071595
AGTACCG800.0044882339.5071595
GTTAGAC1002.735501E-49.5069083
ATTAGAC1304.1995063E-69.5069073
ATTACTC1106.782634E-59.5069073
ACAGCGC900.00111577769.4981098
ATAATAC2252.7284841E-119.2956433
Produced by FastQC (version 0.11.5)