FastQCFastQC Report
Thu 26 May 2016
SRR1512825_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512825_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235421
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52770.23606291611289326No Hit
GTCCTACAGTGGACATTTCTAAATT44990.2012596284995086No Hit
CTGTAGGACGTGGAATATGGCAAGA41030.1835448445729015No Hit
GTCCTAAAGTGTGTATTTCTCATTT39220.17544793575796236No Hit
CTTTAGGACGTGAAATATGGCGAGG32820.14681798193718318No Hit
GGTATCAACGCAGAGTACTTTTTTT31300.14001836790474814No Hit
TATCAACGCAGAGTACTTTTTTTTT28640.1281190433479868No Hit
GTCCTACAGTGTGCATTTCTCATTT25760.11523556412863618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8000.014.0039844
TCCAACG1650.012.65953918
CCAACGA1155.3660187E-912.385447519
GGCGAGG10450.011.72172819
CCACGAA658.03946E-411.68993813
GTAGGAC25500.010.9854963
GTCCTAA12450.010.9699871
TGTAGGA27050.010.9527992
ATAGGAC2650.010.7501173
ATTACAC2250.010.5510413
GGACGTG26100.010.40457256
GTATAAT2600.010.3579011
GGTATCA14450.010.250381
AGGACGT26500.010.2112325
CTGTAGG25400.010.1860471
GTCCTAT2550.010.1838191
TAGGACT3450.010.1822154
TTAGGAC16350.010.1638473
GTATAGG2850.010.1242651
TGGCGAG25000.010.10231318
Produced by FastQC (version 0.11.5)