FastQCFastQC Report
Thu 26 May 2016
SRR1512825_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512825_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235421
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65970.2951121958682503No Hit
GTCCTACAGTGGACATTTCTAAATT47890.21423257632454917No Hit
CTGTAGGACGTGGAATATGGCAAGA44590.19947025638570992No Hit
GTCCTAAAGTGTGTATTTCTCATTT42610.19061286442240635No Hit
GGTATCAACGCAGAGTACTTTTTTT40090.17933982010547453No Hit
CTTTAGGACGTGAAATATGGCGAGG39020.17455324970106303No Hit
TATCAACGCAGAGTACTTTTTTTTT38440.17195866013605493No Hit
GTCCTACAGTGTGCATTTCTCATTT26730.11957479150459802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGA350.00215719116.3008292
TAGGACC7900.013.718514
TTACACG701.08416796E-412.2228834
GGTATCA15500.011.7272991
TATAGCG752.0852145E-411.39373116
TTAGGAC14850.011.2671843
GGCGAGG11700.011.11756619
GATCGTT600.005884447611.08145311
ACGAAAT1855.456968E-1210.78195512
TAGGACA8800.010.6950234
ATAGCGA803.7912856E-410.68162317
GTTAGAC1252.2196582E-710.6474893
AGGACCT17050.010.5941975
TGTAGGA27200.010.5925412
GTAGGAC26500.010.5829143
GACGTGA14200.010.5686297
GTATTAG1909.094947E-1210.5186991
CTGTAGG27250.010.5122651
AGGACGT25450.010.3847475
GGACGTG25450.010.3759156
Produced by FastQC (version 0.11.5)