FastQCFastQC Report
Thu 26 May 2016
SRR1512824_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512824_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2165588
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT63020.291006414885934No Hit
CTGTAGGACGTGGAATATGGCAAGA57590.2659323934192469No Hit
GTATCAACGCAGAGTACTTTTTTTT51120.2360559811007449No Hit
GTCCTAAAGTGTGTATTTCTCATTT50710.23416273086108713No Hit
CTTTAGGACGTGAAATATGGCGAGG45060.20807281902190078No Hit
GTCCTACAGTGTGCATTTCTCATTT38050.17570285760726417No Hit
GGTATCAACGCAGAGTACTTTTTTT31040.14333289619262757No Hit
TATCAACGCAGAGTACTTTTTTTTT27420.12661688188150286No Hit
CTGTAGGACCTGGAATATGGCGAGA26060.12033683230605267No Hit
CTGAAGGACCTGGAATATGGCGAGA23070.106529958607085No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCA400.00527213614.25319216
TATGTCG852.6932867E-713.41476816
TAGGACC11850.013.3840264
TCCAACG2550.012.65831618
CCGAGAA550.003075720312.0874342
CCAACGA1850.011.80439719
AGGACCT23250.011.6006965
GCGTGCG907.66632E-611.5832699
GTGTAGG5050.011.4359411
TGTAGGA35150.011.2940482
GTACTAG1201.06454536E-711.2294591
GGCGAGG15000.011.14061419
GGACCTG23300.011.1271846
CCTAGAC2150.011.0433363
GTTTAGG2450.011.0002871
CGTGCGC951.3788787E-510.98687710
CCAACGT1303.2941898E-810.95558519
GTAGGAC34950.010.8423753
TGTAGAA10250.010.7481282
TGTCGAG1158.895822E-710.73205118
Produced by FastQC (version 0.11.5)