FastQCFastQC Report
Thu 26 May 2016
SRR1512824_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512824_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2165588
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT68880.31806604026250607No Hit
CTGTAGGACGTGGAATATGGCAAGA65490.3024120931589942No Hit
GTATCAACGCAGAGTACTTTTTTTT64400.2973788181316114No Hit
GTCCTAAAGTGTGTATTTCTCATTT54860.2533261174332329No Hit
CTTTAGGACGTGAAATATGGCGAGG50870.23490156022290481No Hit
GTCCTACAGTGTGCATTTCTCATTT41120.18987914598714067No Hit
GGTATCAACGCAGAGTACTTTTTTT41080.18969443864668625No Hit
TATCAACGCAGAGTACTTTTTTTTT38500.17778081518737637No Hit
CTGTAGGACCTGGAATATGGCGAGA30570.1411625849422882No Hit
CTGAAGGACCTGGAATATGGCGAGA27290.126016583025026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTCG453.5444675E-516.8863647
TCCAACG3350.014.73657218
CAGCGTA400.005285231414.2478689
CCGTCAG400.005285231414.2478689
CCAACGT1400.014.2406319
CCAACGA2100.014.01458819
TAGGACC10750.013.6195394
GGACGTA604.0991468E-412.664486
TATGTCG1207.4942363E-1012.65862916
GGCGAGG14150.011.74139119
ACCGTAT658.0357323E-411.6905588
CTAGGAC3750.011.4085753
CGGACAT752.0774164E-411.39829410
TAGGACA8850.011.387094
GTAGGAC33300.011.3057963
TGTAGGA35450.011.2114142
ACCGTCA600.00588346911.0816768
AGGACGT33250.011.0092935
AGGACCG951.3480905E-511.0092935
GTCCTAC36950.011.0008541
Produced by FastQC (version 0.11.5)