FastQCFastQC Report
Thu 26 May 2016
SRR1512823_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512823_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108833
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42290.20053745365327647No Hit
GGTATCAACGCAGAGTACTTTTTTT24400.11570380395223329No Hit
TATCAACGCAGAGTACTTTTTTTTT21700.10290051417063371No Hit
GTCCTACAGTGGACATTTCTAAATT21650.1026634162117152No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGATA350.002172652416.2833413
TCCAACG1450.013.09372418
GTATTAG2550.012.4513411
TAGGACC5850.010.7125514
GGACGTG12300.010.5762236
GTAGGAC14200.010.5658553
AGGACGT12250.010.5418795
TAGGACG12700.010.4671674
TGTAGGA15450.010.3870962
ATTAGGC1106.078566E-610.35915853
CCAACGA1303.8731014E-710.22488619
CAATGCG750.00270012110.1096549
TATTAGG2002.5465852E-119.9706912
GACGTGG7350.09.9476067
TTAGGAC7850.09.919283
CTGTAGG13100.09.9152771
GACGTGA5900.09.8173217
ACCGTGC1356.800583E-79.813178
GTCCTAG2850.09.7902891
GATATAC4450.09.7295971
Produced by FastQC (version 0.11.5)