FastQCFastQC Report
Thu 26 May 2016
SRR1512823_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512823_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108833
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50180.2379515115706175No Hit
GGTATCAACGCAGAGTACTTTTTTT32000.15174269370784693No Hit
TATCAACGCAGAGTACTTTTTTTTT28620.1357148716849556No Hit
GTCCTACAGTGGACATTTCTAAATT23870.11319056558769709No Hit
CTGTAGGACGTGGAATATGGCAAGA21310.10105115009106932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAC550.003073262512.0887057
GTACTAG1209.729774E-911.8985231
CGTTTCC1302.6411726E-911.68546314
TCCAACG1850.011.29068518
TGTGCGG855.3371907E-511.173902510
ATATCCG600.00584234211.0921025
CAACCCG600.005885855811.081059
TAGGACC3550.010.9805124
GGCGAGG6150.010.80667919
CCTAGTA909.419124E-510.5664162
CGTACAC909.4418334E-510.5639073
CCCGTTT1451.4491889E-810.480350512
AGGACCG1002.3786344E-510.4582685
AGGACGT10600.010.4044525
GTCCTAA7100.010.3232261
TGGCGAG11000.010.27125718
GACGTGA5500.010.1890527
GTCTAAC750.002605116510.1534061
TTAGGAC7500.010.141353
GGACCGA750.002651726410.1317266
Produced by FastQC (version 0.11.5)