FastQCFastQC Report
Thu 26 May 2016
SRR1512822_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512822_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075864
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53310.25680873120782477No Hit
GTCCTACAGTGGACATTTCTAAATT42520.20483037424417014No Hit
CTGTAGGACGTGGAATATGGCAAGA37600.18112939961384752No Hit
GTCCTAAAGTGTGTATTTCTCATTT35420.17062774825325744No Hit
GGTATCAACGCAGAGTACTTTTTTT31960.1539599896717704No Hit
TATCAACGCAGAGTACTTTTTTTTT30200.1454815922430371No Hit
CTTTAGGACGTGAAATATGGCGAGG29940.1442291017137924No Hit
GTCCTACAGTGTGCATTTCTCATTT25470.1226958991533164No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAG400.0052969414.2430717
TAGGACC7300.014.0476214
CCAACGA1251.4279067E-912.15143619
TCCAACG2250.011.81189218
TGTAGGA24800.010.7974292
AGGACCT15200.010.7447735
GAAATGT6400.010.5341946
GGCGAGG9400.010.50323619
GTAGGAC22750.010.4764783
TGGCGAG21950.010.37816718
AAATGTC6150.010.3445637
GGTATCA13100.010.2851371
GGACCTG15150.010.2790786
GTCCTAG3750.010.2655461
GTCCTAC20750.010.2500861
TTCGCCT750.002646627110.13405817
AATGTCC5900.010.1023968
GCCTTAC1658.267307E-99.9155851
TCCTACA22800.09.9120862
AGGACGT21800.09.8873935
Produced by FastQC (version 0.11.5)