FastQCFastQC Report
Thu 26 May 2016
SRR1512822_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512822_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075864
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62890.3029581899392253No Hit
GTCCTACAGTGGACATTTCTAAATT46540.22419580473479958No Hit
CTGTAGGACGTGGAATATGGCAAGA43500.20955130008516934No Hit
GGTATCAACGCAGAGTACTTTTTTT41380.1993386850005588No Hit
TATCAACGCAGAGTACTTTTTTTTT40460.19490679543553913No Hit
GTCCTAAAGTGTGTATTTCTCATTT36790.1772274098881237No Hit
CTTTAGGACGTGAAATATGGCGAGG35180.16947160314933926No Hit
GTCCTACAGTGTGCATTTCTCATTT28350.1365696404003345No Hit
CTGTAGGACCTGGAATATGGCGAGA21410.10313777781203393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAG604.0998473E-412.66417611
AACCGTG905.422371E-712.6638717
ACCGTCC550.003074119612.088248
CTCGAAC1650.012.08416418
TAGGACC8250.011.8697164
GGACCGT855.3339827E-511.1745426
TCGAACT1701.2732926E-1111.17023619
TGTAGGA23850.011.002422
AGGACCG700.001481943510.8657455
GTCCTAT3250.010.5441321
CTGTAGG24000.010.4312471
GGCGAGG8850.010.4066219
GTCCTAA10900.010.3049821
GGTATCA16450.010.3002031
TGGCGAA1201.5351015E-610.28592518
GTATTAA3150.010.2744861
GTAGGAC23500.010.2350233
TAAGACG750.00263139510.1411174
AGGCGTG1502.4538167E-810.1310977
CCTATAC2750.010.02539353
Produced by FastQC (version 0.11.5)