FastQCFastQC Report
Thu 26 May 2016
SRR1512821_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512821_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927888
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT47870.24830280597213117No Hit
GTATCAACGCAGAGTACTTTTTTTT45800.23756566771513699No Hit
CTGTAGGACGTGGAATATGGCAAGA41530.21541707817051614No Hit
GTCCTAAAGTGTGTATTTCTCATTT40120.20810337529980996No Hit
CTTTAGGACGTGAAATATGGCGAGG36550.1895857020739794No Hit
GTCCTACAGTGTGCATTTCTCATTT29540.15322466865295079No Hit
GGTATCAACGCAGAGTACTTTTTTT26770.13885661407716632No Hit
TATCAACGCAGAGTACTTTTTTTTT24580.1274970330226652No Hit
CTGTAGGACCTGGAATATGGCGAGA20820.10799382536744874No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCG350.002168195816.28824414
TAGGACC10000.012.4391354
AAATGTC6700.011.6213867
AATGTCC7100.011.5920758
GTGTAGG3700.011.4407351
TAGAAAT7850.011.2494734
GGCGAGG11800.011.18248319
AGGACCT17150.011.12857155
AATGGCG600.00586286411.08679316
GGCGAGT700.001500819710.84919219
GTTCTAA2400.010.8231961
GATATAC4200.010.7659291
TGGCGAG26900.010.763206518
CTCGAAC1158.905263E-710.73098618
CCGTGCA1159.092637E-710.7136489
ACCTTTT7200.010.68916115
TGTAGGA29500.010.6229412
CCACCTT6800.010.61596913
GGACCTG16850.010.594416
GTATAAA2950.010.4359251
Produced by FastQC (version 0.11.5)