FastQCFastQC Report
Thu 26 May 2016
SRR1512821_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512821_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927888
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57200.29669773347829337No Hit
GTCCTACAGTGGACATTTCTAAATT53870.2794249458474766No Hit
CTGTAGGACGTGGAATATGGCAAGA47390.24581303478210353No Hit
GTCCTAAAGTGTGTATTTCTCATTT44020.22833276621878448No Hit
CTTTAGGACGTGAAATATGGCGAGG43320.2247018498999942No Hit
GGTATCAACGCAGAGTACTTTTTTT36260.1880814653133377No Hit
TATCAACGCAGAGTACTTTTTTTTT33090.17163860141253018No Hit
GTCCTACAGTGTGCATTTCTCATTT32530.16873386835749793No Hit
CTGTAGGACCTGGAATATGGCGAGA25160.1305055065439486No Hit
CTGAAGGACCTGGAATATGGCGAGA21350.11074294772310425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9600.013.6667254
CGTTGAA500.001501088113.2984569
CCAACGT802.0155967E-613.05353119
GCCTAAC701.07213506E-412.2373231
GGCGAGG11750.011.95774119
TGGCGAG26450.011.48549118
TCCAACG1850.011.28953918
AGGACGT26350.011.2219985
GGACGTG25600.011.1686256
CTAGGAC3350.011.0676093
AGGACCT18000.011.0398765
GGACCTG17650.010.8712596
GGACCGT700.00149312910.8558826
TTAGGAC16100.010.7468093
AATCCCG2400.010.68016119
TGTAGGA29600.010.6328392
ATGGCGA29150.010.58449417
TAGAAAT6650.010.5795364
GTCCTAA13950.010.5754641
CGTGCGC909.528083E-510.5543310
Produced by FastQC (version 0.11.5)