FastQCFastQC Report
Thu 26 May 2016
SRR1512820_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512820_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050010
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49580.24185247876839625No Hit
GTCCTACAGTGGACATTTCTAAATT32160.15687728352544622No Hit
GGTATCAACGCAGAGTACTTTTTTT30460.14858464105053146No Hit
TATCAACGCAGAGTACTTTTTTTTT28030.1367310403363886No Hit
CTGTAGGACGTGGAATATGGCAAGA27340.13336520309657024No Hit
GTCCTAAAGTGTGTATTTCTCATTT26150.12756035336412994No Hit
CTTTAGGACGTGAAATATGGCGAGG23610.11517016990161023No Hit
GTCCTACAGTGTGCATTTCTCATTT21420.10448729518392594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCGT400.005298298314.2425096
TAGGACC8200.012.736894
CCAACGA1352.7284841E-1112.6565919
GACTGCG802.8821316E-511.8690497
GGTATCA11100.011.7019151
TGTCGAG1001.951801E-611.38953518
GATATAC7050.011.3275271
TCCAACG2650.011.10300518
GGCGAGG8700.010.91085419
TGCCGGT1951.8189894E-1210.70994910
TGTAGGA20950.010.4699472
GTAGGAC20250.010.4098513
GGACGTG17600.010.3581886
GTATCAA24800.010.3199141
AGGACGT18050.010.3101145
GCCGGTT1951.4551915E-1110.22338311
AGGACCT14050.010.2043475
TTAGGAC12200.010.1959573
TGGCGAG19000.010.19063718
AATCCCG2900.010.14709419
Produced by FastQC (version 0.11.5)