FastQCFastQC Report
Thu 26 May 2016
SRR1512819_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512819_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228472
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41780.34009729159476165No Hit
GTCCTAAAGTGTGTATTTCTCATTT29170.23744944939730006No Hit
GTCCTACAGTGGACATTTCTAAATT28300.23036748090310566No Hit
GGTATCAACGCAGAGTACTTTTTTT28020.22808822667508907No Hit
CTGTAGGACGTGGAATATGGCAAGA27830.22654158987750636No Hit
CTTTAGGACGTGAAATATGGCGAGG25320.2061097037620719No Hit
TATCAACGCAGAGTACTTTTTTTTT25260.20562129214178265No Hit
GTCCTACAGTGTGCATTTCTCATTT15140.12324253218632578No Hit
CTGAAGGACCTGGAATATGGCGAGA13790.1122532707298172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGCG400.00536728814.214119
CCGTGTC500.001527985913.2665039
GGTATCA8850.012.2827551
TGCGGTT550.00308534112.08170612
TAGGACC4650.012.0475494
GTCTTAG1104.222038E-711.3687131
TAGACAG1408.40555E-910.8515465
GGACCGA700.001497431410.8515466
GTTCTAT1251.8829633E-710.7740411
GTGTAGG2350.010.6430511
AATCCCG1252.2497807E-710.63582319
GTTTTAA1451.1857992E-810.614821
GTTCTAA1657.4578566E-1010.4941961
TAACCTG1002.4108911E-510.44461255
GTATAGT1201.2961973E-610.421321
TTAGGAC10150.010.38423253
GGCGAGG8700.010.36949119
GTGTAAG1303.2307253E-710.3596551
AGGACGT17400.010.3136455
GACGTGA9500.010.2951127
Produced by FastQC (version 0.11.5)