FastQCFastQC Report
Thu 26 May 2016
SRR1512819_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512819_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228472
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52100.424104090284516No Hit
GGTATCAACGCAGAGTACTTTTTTT35980.2928841683001322No Hit
TATCAACGCAGAGTACTTTTTTTTT34030.2770107906407309No Hit
GTCCTAAAGTGTGTATTTCTCATTT31830.2591023645634577No Hit
GTCCTACAGTGGACATTTCTAAATT31430.25584628709486257No Hit
CTTTAGGACGTGAAATATGGCGAGG30740.25022955346153597No Hit
CTGTAGGACGTGGAATATGGCAAGA30430.24770609342337474No Hit
GTCCTACAGTGTGCATTTCTCATTT16450.13390618589597483No Hit
CTGAAGGACCTGGAATATGGCGAGA15220.1238937476800448No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14900.12128888570516871No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12600.10256644026074668No Hit
ATTTAGAAATGTCCACTGTAGGACG12310.10020578409601522No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCCG602.5519188E-514.2591385
ACACCGT400.005287205314.24636656
TTAACCT551.9489958E-413.8270444
ATCCCGT1205.2750693E-1113.45490210
GGACGAT500.001502118713.2966086
GGTATCA11250.012.7780331
TAGGACC4900.012.2221184
CCAACGA1251.4206307E-912.15393119
TTAGTAC802.8464736E-511.8826163
TCCAACG2000.011.86907318
GTCTAGA907.28381E-611.6356261
CTTATAC1655.456968E-1211.5394651
ATAGGGC752.0608302E-411.40731053
CTAACAT1001.913486E-611.40731053
TTACACC855.2858824E-511.1836384
GGCGAGG8100.011.13641419
GGCACCG600.005846511.0904415
TTATACC600.005846511.0904414
AACACCG600.005846511.0904415
ACTATCC600.005885629411.0805078
Produced by FastQC (version 0.11.5)