FastQCFastQC Report
Thu 26 May 2016
SRR1512818_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512818_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810860
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48690.2688777707829429No Hit
GTCCTACAGTGGACATTTCTAAATT36830.20338402747865653No Hit
CTGTAGGACGTGGAATATGGCAAGA32380.17881006814441758No Hit
GTCCTAAAGTGTGTATTTCTCATTT32290.17831306671968014No Hit
CTTTAGGACGTGAAATATGGCGAGG29120.1608075720928178No Hit
GGTATCAACGCAGAGTACTTTTTTT28660.1582673425886043No Hit
TATCAACGCAGAGTACTTTTTTTTT27050.14937653932385717No Hit
GTCCTACAGTGTGCATTTCTCATTT21960.1212683476359299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1203.6379788E-1214.24226718
TAGGACC7050.013.7379864
CGTTTCC1251.4078978E-912.16151314
CCAACGA1103.840796E-812.08501919
CCCGTTT1201.0139047E-811.86525812
GGCGAGG8800.011.54550919
ATCCCGT1406.91216E-1011.52400910
GGTATCA12450.011.3604351
ACACCGA600.005897616511.0779356
AGGACCT12950.010.9248745
TTAGACT2100.010.8518544
TCCCGTT1408.47831E-910.84672911
TGGCGAG20050.010.70241718
GGACCTG12400.010.6440076
GTAGGAC22500.010.6351133
CTAGGAC3050.010.58529953
TTAGGAC12900.010.526173
TGTAGGA23600.010.501532
AATCCCG2550.010.42629119
TAGGTTG1106.0830334E-610.35835
Produced by FastQC (version 0.11.5)