FastQCFastQC Report
Thu 26 May 2016
SRR1512818_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512818_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810860
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58300.3219464784687938No Hit
GTCCTACAGTGGACATTTCTAAATT41340.2282893210960538No Hit
GGTATCAACGCAGAGTACTTTTTTT38970.2152016169113018No Hit
CTGTAGGACGTGGAATATGGCAAGA38870.214649393106038No Hit
TATCAACGCAGAGTACTTTTTTTTT36420.20111990987707498No Hit
GTCCTAAAGTGTGTATTTCTCATTT35210.19443800183338306No Hit
CTTTAGGACGTGAAATATGGCGAGG32650.18030107241862983No Hit
GTCCTACAGTGTGCATTTCTCATTT23040.12723236473277888No Hit
CTGTAGGACCTGGAATATGGCGAGA18970.10475685585854236No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCGT350.002172917416.2828716
CCAACGA1203.6379788E-1214.24200419
TAGGACC7700.013.8279954
TCCAACG2000.013.76727118
GGTATCA14300.012.1794491
CTTAGAC1302.586603E-911.7006123
CCAACGT1001.943039E-611.39360319
GTAGGAC24600.010.7047523
AGGACCG803.7456985E-410.6953875
TGTAGGA25550.010.6055682
GGCGAGG8600.010.598701519
CTGTAGG24500.010.4884241
CGAAATC1002.4043278E-510.44788713
TAGGACT3100.010.4267554
GATATAC5850.010.4120611
GGACGTG22450.010.4079846
AGGACGT22850.010.3599375
GACGTGG11950.010.2534417
GTCCTAC19700.010.1936291
CTGTCGC750.00265176810.1315649
Produced by FastQC (version 0.11.5)