FastQCFastQC Report
Thu 26 May 2016
SRR1512817_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512817_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709909
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52390.30639057400130654No Hit
GTCCTACAGTGGACATTTCTAAATT39660.2319421676826077No Hit
CTGTAGGACGTGGAATATGGCAAGA35220.20597587356988No Hit
GTCCTAAAGTGTGTATTTCTCATTT32920.19252486535833194No Hit
GGTATCAACGCAGAGTACTTTTTTT31940.18679356620732449No Hit
CTTTAGGACGTGAAATATGGCGAGG29330.1715295960194373No Hit
TATCAACGCAGAGTACTTTTTTTTT29210.17082780428666086No Hit
GTACATGGGAAGCAGTGGTATCAAC22530.13176139782877333No Hit
GTCCTACAGTGTGCATTTCTCATTT20510.11994790366037023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7200.014.1105124
GTACCGT551.9692282E-413.8107986
TGTCGAG1153.8198777E-1013.2072218
TCCAACG1352.7284841E-1112.656919518
TATGTCG1251.4078978E-912.16173516
TGTGCGA550.003085711312.0819610
TGTAGGA21350.011.6521922
CCAACGA907.5110365E-611.60422419
ATGTCGA1406.8030204E-1011.53566217
GTGTTAT2050.011.2611511
GTAGGAC21200.011.1075963
AGGACCT14450.011.1047915
TGTACCG600.00589946111.0774125
AAGGCGT951.3683721E-510.9941226
GGCGAGG9000.010.86577419
AGGACGT19850.010.8581755
CTACACT3000.010.7609144
GACGTGG9800.010.7544557
TCGAACT1158.8840716E-710.73276119
GGACCTG14250.010.7275996
Produced by FastQC (version 0.11.5)