FastQCFastQC Report
Thu 26 May 2016
SRR1512817_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512817_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709909
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64070.37469830265821163No Hit
GTCCTACAGTGGACATTTCTAAATT42890.2508320618231731No Hit
CTGTAGGACGTGGAATATGGCAAGA40570.2372640883228289No Hit
GGTATCAACGCAGAGTACTTTTTTT40000.23393057759214086No Hit
TATCAACGCAGAGTACTTTTTTTTT39400.23042161892825874No Hit
GTCCTAAAGTGTGTATTTCTCATTT35520.20773035290182107No Hit
CTTTAGGACGTGAAATATGGCGAGG34650.20264236283919204No Hit
GTACATGGGAAGCAGTGGTATCAAC24760.1448030275295352No Hit
GTCCTACAGTGTGCATTTCTCATTT21480.12562072016697964No Hit
CTGAAGGACCTGGAATATGGCGAGA19510.1140996392205667No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18370.10743261775919069No Hit
CTGTAGGACCTGGAATATGGCGAGA17250.10088256158661076No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC602.5733718E-514.24716910
TCCAACG1001.4536636E-712.34285318
CAGTCGC550.003072557512.08886059
TAGGACC7500.011.7890424
GCCGGTT1001.9343752E-611.39773611
GTGTAGG2950.011.2924051
GCGTGCG600.005883477611.0814559
GCGCCAC1303.2840035E-810.957437513
GTCCTAA10550.010.9162461
GGCGTGC700.001493643210.8553048
GTCTAGG1605.2568794E-1010.707621
GGCGAGG9450.010.64991819
TGTAGGA24150.010.5920742
TCTACAC2100.010.4127483
TCGAACT1106.0871025E-610.35763819
TGCGCCA1409.7188604E-810.17625112
AGGACCT14350.010.1372885
CGCCACC1502.4683686E-810.12687614
GTGCTAG1701.5079422E-910.077761
GTAGGAC23700.010.0689053
Produced by FastQC (version 0.11.5)