FastQCFastQC Report
Thu 26 May 2016
SRR1512816_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512816_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1906052
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT62050.32554201039635855No Hit
CTGTAGGACGTGGAATATGGCAAGA54300.2848820493879496No Hit
GTCCTAAAGTGTGTATTTCTCATTT53000.2780616688317003No Hit
CTTTAGGACGTGAAATATGGCGAGG46770.24537630662752116No Hit
GTATCAACGCAGAGTACTTTTTTTT42660.22381341117661008No Hit
GTCCTACAGTGTGCATTTCTCATTT33380.17512638689815388No Hit
GTACATGGGAAGCAGTGGTATCAAC25850.13562064413772554No Hit
GGTATCAACGCAGAGTACTTTTTTT24590.12901012144474547No Hit
CTGTAGGACCTGGAATATGGCGAGA23890.12533760883753434No Hit
CTGAAGGACCTGGAATATGGCGAGA23370.12260945661503463No Hit
TATCAACGCAGAGTACTTTTTTTTT22040.11563168266133347No Hit
GTCCTTCAGTGTGCATTTCTCATTT19800.10387964231825784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1058.913048E-1114.46770219
TAGGACC11550.014.1396374
TCCAACG1650.013.80789318
GTGTAGG3850.012.2474241
GGACCGT550.003081161812.0844586
GGCGAAA951.0475542E-611.99296519
GGCGAGG15000.011.96298219
TGGCGAA1353.765308E-1011.95405518
CTAGGAC4250.011.8419893
ACTGCGA658.303201E-411.6448728
TGTAGGA33650.011.4854242
CGTTTCC1001.928409E-611.4008414
GTTCTAA2650.011.2570671
AGGACCT22800.011.2437015
TACGGTG600.0059010111.0771275
GGACCTG21550.011.0150016
GTAGGAC34450.010.9981933
AATCCCG2850.010.9935519
GGACGTG32100.010.9147336
GTCCTAA15000.010.906041
Produced by FastQC (version 0.11.5)