FastQCFastQC Report
Thu 26 May 2016
SRR1512816_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512816_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1906052
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT64390.3378186953976072No Hit
CTGTAGGACGTGGAATATGGCAAGA60880.3194036678957342No Hit
GTCCTAAAGTGTGTATTTCTCATTT56170.294692904495785No Hit
CTTTAGGACGTGAAATATGGCGAGG52930.2776944175709792No Hit
GTATCAACGCAGAGTACTTTTTTTT51550.2704543212881915No Hit
GTCCTACAGTGTGCATTTCTCATTT35480.1861439247197873No Hit
GGTATCAACGCAGAGTACTTTTTTT32970.1729753437996445No Hit
TATCAACGCAGAGTACTTTTTTTTT30560.16033140753767475No Hit
GTACATGGGAAGCAGTGGTATCAAC29590.15524235435339645No Hit
CTGAAGGACCTGGAATATGGCGAGA27700.14532657031392637No Hit
CTGTAGGACCTGGAATATGGCGAGA26540.13924069227911937No Hit
ATTTAGAAATGTCCACTGTAGGACG20620.1081817285152766No Hit
GAATATGGCAAGAAAACTGAAAATC20530.1077095483229209No Hit
GTCCTTCAGTGTGCATTTCTCATTT20410.10707997473311326No Hit
CCCATGTACTCTGCGTTGATACCAC20090.1054011118269596No Hit
TTTCTAAATTTTCCACCTTTTTCAG19470.10214831494628689No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA400.005287235614.2469296
TAGGACC10200.014.0743634
GTATTAA2300.011.588581
TGTAGGA33450.011.5405772
GATATAC5500.011.4230281
CAACCGT752.078439E-411.3975446
CTGTAGG32600.011.3003871
AGGACCT20900.011.2357655
GGCGAGG14000.011.19078419
TGGCGAA855.3515036E-511.17083618
ACAACCG600.00584350211.0917175
GACTGCG600.005885917711.0809457
GTAGGAC32950.011.0219873
GACGTGG15100.010.8817617
ACTGTTC6250.010.7896748
TATGTCG1502.1518645E-910.76095716
AAATGTC8050.010.7368167
AATACGG803.7918857E-410.68127214
GGACGTG31150.010.6413676
GTCGAGG1355.7305442E-810.55023419
Produced by FastQC (version 0.11.5)