FastQCFastQC Report
Thu 26 May 2016
SRR1512815_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512815_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832287
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40370.22032574591207602No Hit
GTCCTACAGTGGACATTTCTAAATT27610.1506860006101664No Hit
GGTATCAACGCAGAGTACTTTTTTT23980.13087469375703695No Hit
CTGTAGGACGTGGAATATGGCAAGA22940.12519872705531393No Hit
GTCCTAAAGTGTGTATTTCTCATTT22420.12236074370445241No Hit
TATCAACGCAGAGTACTTTTTTTTT21650.11815834528106131No Hit
CTTTAGGACGTGAAATATGGCGAGG19110.10429588814416081No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAACG250.006044873518.993243
TAGGACC4400.012.0846174
ACCGTAT550.003160875312.0432688
GTCCTAG2550.011.6955971
GGCGAGG5950.011.48949919
ACACCGT1251.844819E-811.3946926
CTAGTAC1053.4814766E-610.8532823
GTAGGAC14050.010.7470663
TGTAGGA14950.010.6717882
GTATTAT2100.010.5368171
ACTGTTC4200.010.3637658
CTGCGAA1106.2123945E-610.3400669
GGTATCA12700.010.3023441
AGGACGT13250.010.2477765
CCTATAC1303.860241E-710.2271313
GGACGTG13100.010.1479456
TGGCGAG13300.010.06480518
ACCGTCC856.837791E-410.019198
TAGGACG14250.09.9947964
ATGTCGA1054.1014726E-59.95321717
Produced by FastQC (version 0.11.5)