FastQCFastQC Report
Thu 26 May 2016
SRR1512815_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512815_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832287
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47220.2577107188993864No Hit
GGTATCAACGCAGAGTACTTTTTTT28890.15767180578151785No Hit
GTCCTACAGTGGACATTTCTAAATT28280.15434263300454568No Hit
TATCAACGCAGAGTACTTTTTTTTT27960.15259618171170783No Hit
CTGTAGGACGTGGAATATGGCAAGA27230.14861208969992146No Hit
GTCCTAAAGTGTGTATTTCTCATTT24460.13349437069629375No Hit
CTTTAGGACGTGAAATATGGCGAGG22160.12094175202902165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGAC250.006050380418.99025517
GGTATCA12450.011.7703581
ATATCGA600.005884451311.0812796
TAGACTG2450.010.8655145
TACACCG700.00148206310.8655135
CTAGACT1855.456968E-1210.7920974
GGCGAGG7050.010.77461319
GTCCTAT2150.010.6221161
GCTAGAC1451.4262696E-810.490843
GCATTAG1201.491946E-610.3086171
TAGGACC5000.010.267914
TGGCGAG14700.010.20564718
CGTGACC750.00265323310.13090212
TTAGGAC9950.010.0328543
TTAGTAC1151.01709975E-59.9206863
AAGGCGT1151.0287231E-59.9112066
AGGACGT15700.09.8706625
CTAGCAC1651.0089934E-89.7954253
GACGTGA8350.09.78208357
GGACGTG15750.09.7696176
Produced by FastQC (version 0.11.5)