FastQCFastQC Report
Thu 26 May 2016
SRR1512814_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512814_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064140
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46860.22701948511244394No Hit
GGTATCAACGCAGAGTACTTTTTTT28580.13845960060848586No Hit
TATCAACGCAGAGTACTTTTTTTTT24560.11898417742982549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG1302.6375346E-911.6861865
ACCGATG658.3079026E-411.6441728
TAGGACC3950.011.5385424
GACCGTG951.3693312E-510.9937067
TCCAACG1252.2616587E-710.63287418
GTTTAGA2053.6379788E-1210.3252151
GTCCTAC10500.010.2626371
TCCTACA12150.09.7694992
GGTATCA15000.09.6853661
GTATTAC1201.46352995E-59.6212231
GTACTAG1105.9514092E-59.6212231
GTAGGAC11000.09.5822793
TCGTCTC800.0045042099.50313516
TGGACTG3700.09.4950275
GGACCGT900.00111942989.4947956
GCCGGTT900.0011229299.49155511
ACCGTCT900.00115665559.4608898
GGACGTG10000.09.3998476
TGTAGGA12300.09.3415462
TAGAACA5800.09.3315474
Produced by FastQC (version 0.11.5)