FastQCFastQC Report
Thu 26 May 2016
SRR1512814_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512814_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064140
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58820.28496129138527426No Hit
GGTATCAACGCAGAGTACTTTTTTT37870.18346623775519103No Hit
TATCAACGCAGAGTACTTTTTTTTT34680.16801185966068194No Hit
GTACATGGGAAGCAGTGGTATCAAC21630.10478940381950838No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCAC550.00307739912.08651413
GAACCGT752.0777136E-411.3980666
GTAGGAC11450.011.3748093
GACGTGG6100.011.3666487
TAGGACC4700.011.3273874
TGAACCG600.00584291411.0919375
TAAGACG600.005845024311.09144
GGACGTG11050.011.0886166
CCAACGT600.005901197411.07715619
AGGACGT11350.010.973275
CGTGGAA6650.010.8550389
ACGTGGA7100.010.702158
TAGGACG11650.010.6901784
TGTAGGA12900.010.6886592
TAAGCCC1252.2185668E-710.6477444
GATATAC4550.010.4593381
GGACCGT1002.4039136E-510.4482276
CTAAGAC2151.8189894E-1210.169943
GCGTAGA750.0026069410.1525311
GTCCTAC11400.010.0189451
Produced by FastQC (version 0.11.5)