FastQCFastQC Report
Thu 26 May 2016
SRR1512813_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512813_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126003
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59050.2777512543491237No Hit
GGTATCAACGCAGAGTACTTTTTTT35950.16909665696614726No Hit
TATCAACGCAGAGTACTTTTTTTTT30700.1444024302881981No Hit
GTCCTACAGTGGACATTTCTAAATT26410.12422371934564533No Hit
CTGTAGGACGTGGAATATGGCAAGA24320.11439306529670937No Hit
GTCCTAAAGTGTGTATTTCTCATTT23550.11077124538394348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5250.011.93784
GGTATCA15700.011.8230721
GTCCTAA7500.011.7977511
TGGACCG907.4992695E-611.6061955
TGTAGGA16250.010.8702712
GGACCGT1053.4841814E-610.8528036
CTAGACA1855.456968E-1210.7792184
ACTAGAC1355.7172656E-810.5520853
TAGGACT2800.010.5134044
GTAGGAC16250.010.4611743
AGGACGT15350.010.4548545
GTCCTAC14100.010.4362631
GTCTTAG2600.010.3575581
GGACGTG15950.010.2999566
GTGTAGG4300.010.2887361
AGGACCG1201.532504E-610.2873095
ACGAAAT1201.5380974E-610.28438912
TCCTATA2053.6379788E-1210.1917712
TCCTAAA9050.010.1789722
GTCTTAC2105.456968E-1210.075721
Produced by FastQC (version 0.11.5)