FastQCFastQC Report
Thu 26 May 2016
SRR1512813_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512813_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126003
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71890.33814627731005087No Hit
GGTATCAACGCAGAGTACTTTTTTT46340.2179677074773648No Hit
TATCAACGCAGAGTACTTTTTTTTT42160.1983063993794929No Hit
GTCCTACAGTGGACATTTCTAAATT29540.13894618210792742No Hit
CTGTAGGACGTGGAATATGGCAAGA28090.13212587188258906No Hit
GTCCTAAAGTGTGTATTTCTCATTT24680.11608638369748302No Hit
CTTTAGGACGTGAAATATGGCGAGG24380.11467528503017164No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21730.10221058013558776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCCG402.765293E-416.62217910
CTACGAC801.9836461E-613.0722813
TAGGACC5750.012.06990054
TTATACT2500.011.0282514
GGTATCA17350.010.750661
TATACCG803.744273E-410.6960075
ATTAGAC1002.3797706E-510.4578253
GTCCTAC15850.010.3871241
GTAGGAC15150.010.2287763
GTATAAG2250.010.1509621
TGTAGGA16100.010.0988062
TCCTACA16600.09.9664612
TTAGACA2105.456968E-129.9598334
CTGCGCA1151.0287804E-59.9113629
CTACACT2900.09.8349454
CTATACT3000.09.8240184
CTAGAAC2350.09.7093923
GATATAC3650.09.6468911
TTAGGAC9850.09.5553723
TTTGGAC1606.560367E-89.5071133
Produced by FastQC (version 0.11.5)