FastQCFastQC Report
Thu 26 May 2016
SRR1512812_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512812_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2164214
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT57160.2641143620732515No Hit
GTATCAACGCAGAGTACTTTTTTTT55060.25441107025460513No Hit
CTGTAGGACGTGGAATATGGCAAGA49690.22959836688978075No Hit
GTCCTAAAGTGTGTATTTCTCATTT48850.22571705016232224No Hit
CTTTAGGACGTGAAATATGGCGAGG40380.18658043982711506No Hit
GGTATCAACGCAGAGTACTTTTTTT30940.14296183279472363No Hit
GTCCTACAGTGTGCATTTCTCATTT29120.13455231321856342No Hit
TATCAACGCAGAGTACTTTTTTTTT29000.13399783940035503No Hit
GTACATGGGAAGCAGTGGTATCAAC23840.11015546521739533No Hit
CTGAAGGACCTGGAATATGGCGAGA23180.1071058592172493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG350.002166839316.28990216
CCAACGA2150.014.57226219
GGACCGT801.2965211E-714.2433916
TCCAACG3050.014.00598518
GCGTGCG500.001524480513.2713089
CAAGTCG2300.012.798416
TGGACCG751.4866342E-512.6604985
TAGGACC9450.012.3590574
AAGTCGT2500.012.154367
AGTCGTC2500.012.1121218
GTGCGAT752.0911377E-411.39021111
GACCGTG855.350047E-511.1712887
TACGACC600.005898247511.0779364
GTCCTAA14250.010.7351011
AGGACCT19450.010.5449915
CCACCTT7900.010.45838913
GGACCTG18650.010.4375166
AATGTCC7200.010.3825698
CACCTTT8000.010.21346314
TAGAAAT8100.010.1987354
Produced by FastQC (version 0.11.5)