FastQCFastQC Report
Thu 26 May 2016
SRR1512812_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512812_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2164214
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66700.3081950306208166No Hit
GTCCTACAGTGGACATTTCTAAATT61650.2848609241045479No Hit
CTGTAGGACGTGGAATATGGCAAGA57410.26526951586118563No Hit
GTCCTAAAGTGTGTATTTCTCATTT53420.24683326140575748No Hit
CTTTAGGACGTGAAATATGGCGAGG46620.2154130783739501No Hit
GGTATCAACGCAGAGTACTTTTTTT43090.19910230688832067No Hit
TATCAACGCAGAGTACTTTTTTTTT41180.19027693194850417No Hit
GTCCTACAGTGTGCATTTCTCATTT31000.14323906970382783No Hit
CTGAAGGACCTGGAATATGGCGAGA27080.1251262583090212No Hit
GTACATGGGAAGCAGTGGTATCAAC26260.12133735388459738No Hit
CTGTAGGACCTGGAATATGGCGAGA24900.11505331727823588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1650.014.38497719
TCCAACG2450.014.33800518
CCCGTTT1850.013.34951512
TAAGACG500.001491783113.3095564
TAGGACC8800.012.9638544
ATCCCGT1900.012.498569510
TGGACCG657.9743395E-411.701255
GCCGGTT1406.82121E-1011.53466211
TGTAGGA30650.011.19805052
AGGACGT29350.011.045925
GTATAAG2950.010.9679311
GGACGTG29250.010.8793496
CCGTTTC2100.010.85364313
TGGCGAA1408.438292E-910.85038318
TAGGACT4250.010.7371214
GTAGGAC30650.010.7315163
CTGTAGG30900.010.6865821
GACGTGG15600.010.5951867
CCGGTTT1451.447188E-810.481317512
GACGTGA15000.010.4490467
Produced by FastQC (version 0.11.5)