FastQCFastQC Report
Thu 26 May 2016
SRR1512811_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512811_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747380
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35920.20556490288317367No Hit
GTCCTACAGTGGACATTTCTAAATT30130.17242958028591376No Hit
CTGTAGGACGTGGAATATGGCAAGA27130.15526101935469103No Hit
GTCCTAAAGTGTGTATTTCTCATTT26670.1526285066785702No Hit
CTTTAGGACGTGAAATATGGCGAGG22560.12910757820279506No Hit
GGTATCAACGCAGAGTACTTTTTTT21900.12533049479792605No Hit
GTACATGGGAAGCAGTGGTATCAAC20220.11571610067644131No Hit
TATCAACGCAGAGTACTTTTTTTTT18720.10713182021082993No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG359.980358E-518.9900915
TAGGACC6600.013.5236374
ACCGTAT550.003159300712.0440198
AGGACGT18250.011.9143325
TATGTCG802.8601431E-511.87770316
GGACGTG18100.011.6989916
TAAAACG658.057492E-411.6865474
GGCGAGG7400.011.67701319
AGGACCT12500.011.6219365
GACGTGG9000.011.3940557
TTAGGAC10850.011.2906683
GTAGGAC19100.011.2365943
TGTAGGA19700.011.2317982
GGACCTG12350.011.2255596
TAGGACG19500.011.1022214
TATACCG600.005898986511.0775545
GACGTGA9350.011.0690927
GTCCTAC14950.010.7438991
GCCTAAT908.395263E-510.6867131
CAATACA2050.010.6532854
Produced by FastQC (version 0.11.5)