FastQCFastQC Report
Thu 26 May 2016
SRR1512811_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512811_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747380
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44150.2526639883711614No Hit
GTCCTACAGTGGACATTTCTAAATT32510.18604997195801715No Hit
CTGTAGGACGTGGAATATGGCAAGA31720.18152891757946182No Hit
GTCCTAAAGTGTGTATTTCTCATTT28890.16533324176767503No Hit
CTTTAGGACGTGAAATATGGCGAGG26980.1544025913081299No Hit
TATCAACGCAGAGTACTTTTTTTTT26510.15171285009557164No Hit
GGTATCAACGCAGAGTACTTTTTTT26370.15091165058544792No Hit
GTACATGGGAAGCAGTGGTATCAAC23970.13717680184046974No Hit
GTCCTACAGTGTGCATTTCTCATTT19400.11102336068857376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6550.012.3374864
TCCAACG1406.87578E-1011.52916318
AACCGTG752.0773202E-411.3980927
CGCCACC600.00589979311.07735614
GGCGAGG7200.010.813319
GTCTAGA1158.626539E-710.7573541
GTTCTAG1605.275069E-1010.7056371
CTAGGAC1605.366019E-1010.6955133
CGTGCGC803.77701E-410.68571310
GGACGTG16350.010.6312486
AGGACGT16650.010.6211775
TGTAGGA19000.010.6094672
GTAGGAC18150.010.5809293
TAAGCCC909.443678E-510.5634684
GTCTAGG1553.5615813E-910.4370361
TAGGACG17200.010.2256834
TTAGGAC9200.010.1271513
GACGTGG8950.010.0821127
GTCCTAA9950.010.0421381
GATATAC3800.010.0169711
Produced by FastQC (version 0.11.5)