FastQCFastQC Report
Thu 26 May 2016
SRR1512810_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512810_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1564753
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39070.2496879699224095No Hit
GGTATCAACGCAGAGTACTTTTTTT23790.15203677513319994No Hit
TATCAACGCAGAGTACTTTTTTTTT21760.1390634815846335No Hit
GTACATGGGAAGCAGTGGTATCAAC17040.10889897638796667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1353.7471182E-1011.95479918
GTCTTAG1655.456968E-1211.6637691
TAGACAG2500.011.0138935
GTGTTAG2050.010.7960991
TTAGACA1855.456968E-1210.7774624
TGTACCG803.7900463E-410.6815785
GTTAGAC1252.2555469E-710.6344453
TAGGACG8800.010.357564
CCCGTTT1201.5378973E-610.28398312
TAGGACA3800.010.2439914
GTATAAC951.4441858E-410.1290621
GGCGAGG4500.010.12771819
TGTAGGA9800.010.0763692
GTAGGAC9150.010.0657823
GACGAAA856.6462194E-410.05002411
GGACGTG8250.010.0129526
TGGCGAG8250.010.01134518
AGGACGT8350.010.0064285
CTTAGAC951.6525123E-49.9947793
GTCATAG1658.278221E-99.9142041
Produced by FastQC (version 0.11.5)