FastQCFastQC Report
Thu 26 May 2016
SRR1512810_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512810_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1564753
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49390.3156408711151217No Hit
GGTATCAACGCAGAGTACTTTTTTT32500.2077005124770491No Hit
TATCAACGCAGAGTACTTTTTTTTT31270.1998398469279177No Hit
GTACATGGGAAGCAGTGGTATCAAC20490.13094718463553034No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16090.10282773063863754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3400.013.9764914
GTACCGT500.001500563513.2988136
GTGTAAC1103.744026E-812.106861
ATAAGAC1353.6925485E-1011.9680183
GACCGTG658.029476E-411.6912657
GTACTAA1001.899054E-611.4150391
GTAGGAC9250.011.3020713
GTTCTAG1803.6379788E-1211.0979561
TAATACC1502.1173037E-910.7712154
GGCGAGG4100.010.65452119
ATTAGGG909.473579E-510.5600163
TTAGGAC5200.010.4178623
AGGACCT6500.010.3819715
TAGGCTC1201.5137703E-610.2966755
GGACGTG7200.010.2907496
GGATTAG1606.035407E-910.1070661
AGGACGT7300.010.0254225
TGTAGGA10850.09.9861042
TAGGACA4950.09.9840154
GAACAGT3650.09.8895296
Produced by FastQC (version 0.11.5)