FastQCFastQC Report
Thu 26 May 2016
SRR1512809_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512809_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875432
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46720.2491159370214436No Hit
GTCCTACAGTGGACATTTCTAAATT40720.21712330812314176No Hit
GTCCTAAAGTGTGTATTTCTCATTT35830.19104931557102578No Hit
CTGTAGGACGTGGAATATGGCAAGA34390.18337108463543333No Hit
CTTTAGGACGTGAAATATGGCGAGG30170.16086960231029437No Hit
GGTATCAACGCAGAGTACTTTTTTT26720.1424738406937708No Hit
TATCAACGCAGAGTACTTTTTTTTT25110.13388915193939316No Hit
GTCCTACAGTGTGCATTTCTCATTT21360.11389375887795451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8050.012.2675394
TACACCG550.003079633712.0852415
GTGTAGG4550.011.8408851
TAGAACG658.05636E-411.6868274
TAGGACT2700.011.6056684
GTATTAA2200.010.9326361
GCCTAAG1157.494036E-710.8755961
TTAGACA1752.0008883E-1110.8520544
GTCCTAA13400.010.8412581
GGCGAGG10000.010.72939219
TATACTG3100.010.7207785
TGTAGGA24800.010.3001582
CCTAGAC1201.5319747E-610.2873953
GTAGAAC6750.010.2698093
AGGACCT15500.010.1694235
GCAATTC750.002654765710.13021413
TGTAGAA7600.010.1207962
GAACAGT4800.010.089296
GTAGGAC23950.09.9916753
TCTAGAC2002.5465852E-119.9708593
Produced by FastQC (version 0.11.5)