FastQCFastQC Report
Thu 26 May 2016
SRR1512809_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512809_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875432
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56770.3027035904260992No Hit
GTCCTACAGTGGACATTTCTAAATT43010.2293338281526603No Hit
CTGTAGGACGTGGAATATGGCAAGA40190.21429729257045846No Hit
GTCCTAAAGTGTGTATTTCTCATTT39430.2102448929100069No Hit
GGTATCAACGCAGAGTACTTTTTTT36380.19398197322003677No Hit
CTTTAGGACGTGAAATATGGCGAGG35510.189343042029783No Hit
TATCAACGCAGAGTACTTTTTTTTT33830.1803851059382585No Hit
GTCCTACAGTGTGCATTTCTCATTT23160.12349154754744507No Hit
CTGAAGGACCTGGAATATGGCGAGA19450.10370943867866177No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAT550.003071530712.089510
GACCGCA550.003071530712.08957
GTGTACG657.9165143E-411.7112531
TAAGACG657.975279E-411.7009344
TAGGACC7600.011.3833914
ACCGTCC600.005881523711.0820428
TGTCGAG951.3695248E-510.9934418
AATCCCG2100.010.85066819
AGACGTG803.7736574E-410.6868256
GGTATCA14900.010.6010421
AGGACCT14000.010.526465
CACCTTT6550.010.43735514
GGCGAGG9650.010.42900919
CTAAGGC1553.61797E-910.4270423
GTCCTAC21500.010.2677731
CTATACT2500.010.267574
GGACCTG13900.010.2511516
CCTATAC1303.8030157E-710.23831753
ACCTTTT6900.010.182615
GTCCTAA11900.010.1550841
Produced by FastQC (version 0.11.5)