FastQCFastQC Report
Thu 26 May 2016
SRR1512808_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512808_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2127482
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT70500.3313776567792348No Hit
GTCCTAAAGTGTGTATTTCTCATTT61960.29123630658214733No Hit
CTGTAGGACGTGGAATATGGCAAGA61140.2873819849004598No Hit
CTTTAGGACGTGAAATATGGCGAGG55750.26204687043180624No Hit
GTATCAACGCAGAGTACTTTTTTTT49960.2348315990452563No Hit
GTCCTACAGTGTGCATTTCTCATTT38530.1811061151163676No Hit
GGTATCAACGCAGAGTACTTTTTTT29570.13899060015548897No Hit
CTGAAGGACCTGGAATATGGCGAGA29500.1386615726948571No Hit
TATCAACGCAGAGTACTTTTTTTTT26740.1256884899613722No Hit
CTGTAGGACCTGGAATATGGCGAGA26480.12446638796473954No Hit
GTACATGGGAAGCAGTGGTATCAAC25470.11971899174705121No Hit
GTCCTTCAGTGTGCATTTCTCATTT23380.10989517185104268No Hit
ATTTAGAAATGTCCACTGTAGGACG23140.10876707770030487No Hit
TTTCTAAATTTTCCACCTTTTTCAG21990.10336162656135281No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGT552.0301927E-413.7615368
TAGGACC10750.013.2502014
AATGGCG1157.0565875E-811.56771116
TCCAACG2700.011.25209518
CTACACT4300.011.0418354
ACCTTTT8700.011.03006815
GACGGGG951.3674862E-510.9950827
GGCGAGG16950.010.86700919
TGTCGAG1408.438292E-910.85023518
TCAATAC1158.860443E-710.7353463
CACCTTT8950.010.72196614
CTACACG803.786674E-410.6829754
GTGTAGG5500.010.6682111
GTCCTAC34250.010.6158721
AGGACCT20850.010.6115615
CCACCTT9300.010.51904213
TAGAAAT10300.010.5101114
GCGCAGA650.00970756610.3587921
GGACCTG20800.010.3177446
CCTACAC3500.010.310653
Produced by FastQC (version 0.11.5)