FastQCFastQC Report
Thu 26 May 2016
SRR1512808_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512808_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2127482
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT76840.3611781439278922No Hit
CTGTAGGACGTGGAATATGGCAAGA71130.3343389039249216No Hit
GTCCTAAAGTGTGTATTTCTCATTT67070.31525531120827344No Hit
CTTTAGGACGTGAAATATGGCGAGG63700.29941498917499654No Hit
GTATCAACGCAGAGTACTTTTTTTT61670.28987319281667245No Hit
GTCCTACAGTGTGCATTTCTCATTT42120.19798052345448752No Hit
GGTATCAACGCAGAGTACTTTTTTT38320.18011903273447202No Hit
TATCAACGCAGAGTACTTTTTTTTT34640.16282158908982544No Hit
CTGAAGGACCTGGAATATGGCGAGA33490.1574161379508734No Hit
CTGTAGGACCTGGAATATGGCGAGA31970.1502715416628672No Hit
GTACATGGGAAGCAGTGGTATCAAC27270.12817969787758485No Hit
GTCCTTCAGTGTGCATTTCTCATTT25150.11821486621273411No Hit
TTTCTAAATTTTCCACCTTTTTCAG24990.11746280344557558No Hit
ATTTAGAAATGTCCACTGTAGGACG24910.11708677206199629No Hit
GAATATGGCAAGAAAACTGAAAATC23270.1093781286986212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2200.014.67288118
TAGGACC13450.013.9263254
CGTGCGC500.001501963713.29753110
CCAACGA1800.013.18641319
GCGCCAC905.429574E-712.66252813
CTAGGAC3700.012.33483
TACACCG550.003048541312.1017565
TCGCATT550.00308311212.083549518
GGCGAGG16750.011.84651619
ACCTTTT9050.011.74864515
GACGTGG18850.011.6400227
CTAGTAC1001.9108575E-611.4096913
TGTAGGA40200.011.4007652
AGGACCT24500.011.3714175
CGCATTT855.357893E-511.16966719
GTAGGAC39750.011.1465733
TATTGCG700.001499684510.85027916
AAATGTC8150.010.8387347
CCACCTT9300.010.82441913
GGACCTG24400.010.8217616
Produced by FastQC (version 0.11.5)