FastQCFastQC Report
Thu 26 May 2016
SRR1512807_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512807_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621134
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41350.25506836572423996No Hit
GGTATCAACGCAGAGTACTTTTTTT25150.15513831675851594No Hit
TATCAACGCAGAGTACTTTTTTTTT22610.139470272044137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAC853.9611077E-612.2892383
ACACCGT802.8800116E-511.8695496
GGCTAAT803.3610934E-410.82131
GTTCTAG1802.7284841E-1110.6877031
TAGGACC2700.010.5503834
GTCCTAT1201.2984783E-610.420511
GTCCTAC6700.010.1931961
ATAGGAC1701.5679689E-910.0548313
ATTACCC856.617329E-410.0548313
GTAGGAC6150.010.0366653
ATTATAC1901.0004442E-109.9960313
TGGCGAG6800.09.91271918
AGGACGT6650.09.8526185
GGCGAGG3400.09.77340619
TGTAGGA6900.09.77148152
GTGTAGA3700.09.6189321
GTATAAT1408.9297646E-79.6189321
TAGGACG6250.09.5713074
GCACTCG800.0045269349.49711313
TCTATAC1804.2073225E-99.4962293
Produced by FastQC (version 0.11.5)