FastQCFastQC Report
Thu 26 May 2016
SRR1512807_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512807_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621134
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49250.3037996858988831No Hit
GGTATCAACGCAGAGTACTTTTTTT31350.1933831503132992No Hit
TATCAACGCAGAGTACTTTTTTTTT30340.1871529435567942No Hit
GTACATGGGAAGCAGTGGTATCAAC17920.1105399060164058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC5850.011.547511
CCAACGA855.352531E-511.17039219
CCGTTAT600.005809515711.1002481
TAGGACC2700.010.56354
ATAGGAC2100.010.4122723
GTCTAAG1659.0403773E-1010.3794521
GGTATCA14100.010.2567631
TAATCTG1303.7992322E-710.2387865
AGTACCG750.002630524310.1412745
TCCTACA7350.010.0901562
TTATACC856.5478095E-410.0663964
TCCAACG1252.5871796E-69.87462618
GTCCTAA3650.09.64483451
GCCTTAA1502.588895E-79.5144981
GGGTAAG1502.588895E-79.5144981
CCTATCC1201.6855196E-59.5068583
CCAGTAC1804.127287E-99.5068573
TCTATAC1804.127287E-99.5068573
TATACTG2900.09.1796025
CCCTTAT1252.7041564E-59.1339181
Produced by FastQC (version 0.11.5)