FastQCFastQC Report
Thu 26 May 2016
SRR1512806_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512806_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113074
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53780.254510727026124No Hit
GGTATCAACGCAGAGTACTTTTTTT30840.1459485091388186No Hit
TATCAACGCAGAGTACTTTTTTTTT30430.14400820794728436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT1353.765308E-1011.9551686
GGTATCA10550.011.3989511
GTATCAA24750.010.9617821
AGCACCG1451.4575562E-810.4764275
GTCCTAC7450.010.4601051
GTAAGAC1002.4123541E-510.4449313
TATGCCG1002.4155257E-510.4436875
GTCCTAG2300.010.0390061
GTCTTAG1855.14774E-109.88073351
TCCTACA9400.09.7984372
GGCGAGG3800.09.74573519
TGGCGAG7650.09.68042318
GTCTAGC1002.4274559E-49.6207141
GTCTTAT2400.09.6207141
AGGACGT7550.09.55713755
AATCCCG1002.7680074E-49.49584419
ACTGCGT900.00115313639.4640658
ACCGAGT1002.8627692E-49.4640658
ACCGTGC1502.7985698E-79.4640658
TTTAGAA3850.09.3720752
Produced by FastQC (version 0.11.5)