FastQCFastQC Report
Thu 26 May 2016
SRR1512806_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512806_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113074
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65900.31186792322464807No Hit
TATCAACGCAGAGTACTTTTTTTTT40080.18967627257729733No Hit
GGTATCAACGCAGAGTACTTTTTTT39870.18868245977187736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCCG707.2718576E-613.57027910
TAGACCG500.001490935613.31053455
GGTATCA15100.012.4745071
TAGGACC3600.012.4120134
TACCGTA701.09212124E-412.2132517
TAGGACG7250.011.67075354
TTAGGAC5250.011.4084893
GTAGGAC8600.011.3863793
AGGACGT7550.011.2075455
GGACGTG7600.010.9990676
GTTAGGC700.001482550410.8652283
TCGAACT1053.4947607E-610.85005719
GTATCAA30000.010.1793231
CCGTATG750.00265010610.1324759
CCGCGTC750.00265010610.1324759
GACGTGA4150.010.0714367
TTATACT1701.538865E-910.0663144
ACCGTAT856.600172E-410.0579718
CCAATAC1803.8926373E-1010.0352453
CACACGT1151.0176551E-59.9204254
Produced by FastQC (version 0.11.5)