FastQCFastQC Report
Thu 26 May 2016
SRR1512805_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512805_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439880
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47540.3301664027557852No Hit
GGTATCAACGCAGAGTACTTTTTTT27280.18946023279717755No Hit
TATCAACGCAGAGTACTTTTTTTTT25170.17480623385282107No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGCG400.00529548114.24323753
TCAAGCG500.001504818713.2936893
GTCTTAG1550.013.0237071
TGGACCG658.059469E-411.6859435
GCCTTAC854.719051E-511.3091011
GTAAGAC1354.7602953E-911.2539173
GACGTGA3400.011.1699957
ATATACG600.005897599711.0776864
CTACACG600.005897599711.0776864
TAGGACC2500.011.0143854
TGTACCG700.001498066710.8512325
TAAGACA2200.010.7899554
TAGACTG1502.1500455E-910.7608055
GGTATCA9300.010.7497271
CTAGACA1701.4006218E-1010.6122374
GTCCTAT1002.1051155E-510.574011
GTATCAA20550.010.57167151
TAGAGGT1451.4537363E-810.4774174
CTTAGAC1002.410601E-510.4450413
CTACCCT1409.753421E-810.1733864
Produced by FastQC (version 0.11.5)