FastQCFastQC Report
Thu 26 May 2016
SRR1512805_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512805_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439880
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56770.39426896685834933No Hit
GGTATCAACGCAGAGTACTTTTTTT35740.24821512903853096No Hit
TATCAACGCAGAGTACTTTTTTTTT34380.23876989749145763No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16530.11480123343611968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11400.012.9402941
GTCTTAC751.45088E-512.68983651
ACACCGT853.944997E-612.2934886
TAGGACC2500.012.1674134
ACCGTGC658.028574E-411.6913028
ATTACAC1251.814442E-811.4069493
CTATTAA1354.5911293E-911.2798551
GGACCGT600.00587753811.0827666
GGATAAG951.3326173E-511.0201211
ATAGGAC1252.2201311E-710.6464863
GTCCTAC6100.010.6095351
TGGCGAG5550.010.60543318
CTAGGAC2100.010.4111043
TAGGACA3200.010.3969594
TACTACA1855.820766E-1110.2783182
TAGCCTA750.002632496210.1402165
GTAGGAC5800.09.997473
TGGGCCG1151.0177218E-59.9197775
GTATAGG1252.5091958E-69.8980721
GCCTTAT1451.5733167E-79.8455621
Produced by FastQC (version 0.11.5)