FastQCFastQC Report
Thu 26 May 2016
SRR1512804_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512804_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970137
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41340.20983312328025922No Hit
GTACATGGGAAGCAGTGGTATCAAC32570.16531845247310212No Hit
GGTATCAACGCAGAGTACTTTTTTT24570.12471213930807858No Hit
GTCCTACAGTGGACATTTCTAAATT24290.12329091834730274No Hit
CCCATGTACTCTGCGTTGATACCAC22690.11516965571429805No Hit
TATCAACGCAGAGTACTTTTTTTTT21640.1098400771113887No Hit
CTGTAGGACGTGGAATATGGCAAGA20890.10603323525216773No Hit
GTCCTAAAGTGTGTATTTCTCATTT19810.10055138297488957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4700.012.9310234
TCGCCAA550.003067512512.09165814
TCGGAAC550.003078095812.0860983
CCTAATA1001.9394938E-611.3954632
GGACCGT855.3458934E-511.1720236
CTGTTCG600.00597630611.0583049
GACCGTG1053.4836794E-610.8528237
CACACCG803.785512E-410.6832485
GTAGGAC14350.010.6542953
GGACGTG14050.010.5438456
GTCCTAA7600.010.5082991
ATAGGAC2100.010.4006213
AGGACGT14900.010.3884825
TGTAGGA14450.010.38340952
CCAATAC1659.349606E-1010.3595123
GTCTAAG1701.2496457E-910.1880591
TAGGACT3000.010.1293014
GGCGAGG5750.010.07270719
GTCCTAC13350.09.9463951
CCGTGCA1054.20895E-59.9299059
Produced by FastQC (version 0.11.5)