FastQCFastQC Report
Thu 26 May 2016
SRR1512804_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512804_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970137
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51710.262469056720421No Hit
GTACATGGGAAGCAGTGGTATCAAC36950.1875504089309525No Hit
GGTATCAACGCAGAGTACTTTTTTT31720.16100403169931837No Hit
TATCAACGCAGAGTACTTTTTTTTT31180.15826310556067927No Hit
GTCCTACAGTGGACATTTCTAAATT26260.1332902229641898No Hit
CCCATGTACTCTGCGTTGATACCAC25640.1301432336939005No Hit
CTGTAGGACGTGGAATATGGCAAGA22430.11384995053643476No Hit
GTCCTAAAGTGTGTATTTCTCATTT21540.1093324981968259No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG751.4682373E-512.6757155
TAGGACC6150.011.1296144
GTACTAA1553.0195224E-1011.05306151
GTACTAG1303.1777745E-810.9822091
CGTGCGC803.776858E-410.68590110
GTCCTAA6000.010.4697071
TCGAACT1201.5349106E-610.28594719
GACCGGG750.00265139810.1318177
GTCCTAC13500.010.0819391
CCTATAC1803.8926373E-1010.0341763
GTCTAGG2300.09.9317371
TACACCG1252.5469471E-69.8870575
GAAATGT4550.09.8122166
GTAGGAC14650.09.7980573
TCCTATA1651.0086296E-89.7958672
GTCCTAG2450.09.7121581
GACGTGG6800.09.6382637
TGTAGGA15200.09.6328552
AGGACGT12500.09.582845
TCCTACA15000.09.5077532
Produced by FastQC (version 0.11.5)