FastQCFastQC Report
Thu 26 May 2016
SRR1512803_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512803_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550582
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44430.28653757105396555No Hit
GGTATCAACGCAGAGTACTTTTTTT27630.17819115661087256No Hit
TATCAACGCAGAGTACTTTTTTTTT24060.1551675435417153No Hit
GTACATGGGAAGCAGTGGTATCAAC16310.10518631068850276No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT350.002229904816.2196628
GTGTTAG1455.456968E-1212.6052671
CTAGGAC1650.012.0864283
GTCGTGA600.00535204811.2231111
GTATAAG1656.366463E-1111.0773561
GTCCTAA4050.010.6886781
CCTAGAC909.5498544E-510.5516433
GACGTGG3200.010.3864367
GGACGTG6150.010.3454216
TAGGACC1752.3646862E-1010.3101274
TAGGACG6550.010.2935584
GTACTAA855.874979E-410.185681
TAGGACT2151.8189894E-1210.1586934
TACACGC750.002656419810.1292485
GACCCGG750.002666449410.12464711
GTCTTAC1553.344394E-89.9301261
GTCTAAG1951.2914825E-109.8664711
AGGACGT6550.09.8586195
ACTATAC1451.6249032E-79.8239433
CGTGGAA3350.09.6204199
Produced by FastQC (version 0.11.5)