FastQCFastQC Report
Thu 26 May 2016
SRR1512803_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512803_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550582
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48740.3143335857116876No Hit
GGTATCAACGCAGAGTACTTTTTTT30910.1993445041926193No Hit
TATCAACGCAGAGTACTTTTTTTTT30460.196442368091465No Hit
GTACATGGGAAGCAGTGGTATCAAC17720.1142796704721195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2250.013.097814
GTATTAG604.0310255E-412.6892321
GTATTAC1251.371518E-912.1816631
TAGGACT2300.010.7464494
TACACCG803.7458766E-410.6951295
TATACTG1909.094947E-1210.5074965
CTAGGAC1659.1858965E-1010.3703653
GTCTTAT1909.640644E-1110.0178161
GTACTAA1054.0246887E-59.9701121
GTCCTAC6200.09.8239211
TCCTATA1002.7307164E-49.5083172
TTAGGAC4100.09.5061683
TCTATAC1401.0486801E-69.5061673
GGTATCA14600.09.451741
GTTCTAG1856.488335E-99.259711
TCCTACA7250.09.1804452
GTATCAA25100.09.175681
GTAGGAC7050.09.169073
GTCTAAG1356.6911125E-69.1644451
TGGCGAG6150.09.10885418
Produced by FastQC (version 0.11.5)